Journal: Applied Microbiology and Biotechnology
Article Title: Redox regulation of hydrogen production in Escherichia coli during growth on by-products of the wine industry
doi: 10.1007/s00253-025-13535-w
Figure Lengend Snippet: Changes in ORP ( A ) and H 2 production yield in the gas phase ( B ) of E. coli BW25113 and mutant strain in the presence of redox reagents during growth in 2X diluted WGW at pH 7.5. Bacteria were grown anaerobically. ORP was measured with a platinum electrode (Pt) and expressed in mV (Ag/AgCl (saturated with KCl)). An average of 3 independent experiments is presented, p < 0.05. DTT—DL-dithiothreitol, F—potassium ferricyanide, PS—parental strain, MS—septuple mutant
Article Snippet: The ORP of the medium was monitored using redox electrodes: platinum (Pt) (EPB-1, Measuring Instruments Enterprise, Gomel, Belarus, or PT42BNC, Hanna Instruments, Portugal) and titanium-silicate (Ti-Si) (EO-02, measuring instruments enterprise, Gomel, Belarus) electrodes (Poladyan et al. ).
Techniques: Mutagenesis, Bacteria